The references below highlight the versatility of the cell-free split-luciferase platform for detection of various biomolecular targets.  Click on a link below for more information.

Cell-free split-protein systems

1)  Shekhawat, SS; Ghosh, I.  Split-protein systems: beyond binary protein-protein interactions.  Curr. Op. Chem. Biol. 15, 789-797 (2011).

2)  Porter, JR; Stains, CI; Jester, BW; Ghosh, I.  A general and rapid cell-free approach for the interrogation of protein-protein, protein-DNA, and protein-RNA interactions and their antagonists utilizing split-protein reporters.  J. Am. Chem. Soc. 130, 6488-6497 (2008).

KinaseSeeker Assay

3)  Jester, BW; Gaj, A; Shomin, CD; Cox, KJ; Ghosh, I.  Testing the Promiscuity of Commercial Kinase Inhibitors Against the AGC Kinase Group Using a Split-luciferase Screen.  J. Med. Chem. 55, 1526-1537 (2012).

4)  Jester, BW; Cox, KJ; Gaj, A; Shomin, CD; Porter, JR; Ghosh, I.  A Coiled-Coil Enabled Split-Luciferase Three-Hybrid System: Applied Toward Profiling Inhibitors of Protein Kinases.  J. Am. Chem. Soc. 132, 11727-11735 (2010).

Protein-protein Interactions

5)  Stains, CI; Furman, JL; Porter, JR; Rajagopal, S; Li, YX; Wyatt, RT; Ghosh, I.  A General Approach for Receptor and Antibody-Targeted Detection of Native Proteins Utilizing Split-Luciferase Reassembly.  ACS Chem. Biol. 5, 943-952 (2010).

6)  Henchey, LK; Porter, JR; Ghosh, I; Arora, PS.  High Specificity in Protein Recognition by Hydrogen-Bond-Surrogate alpha-Helices: Selective Inhibition of the p53/MDM2 Complex.  ChemBiochem 11, 2104-2107 (2010).

7)  Porter, JR; Helmers, MR; Wang, P; Furman, JL; Joy, ST; Arora, PS; Ghosh, I.  Profiling small molecule inhibitors against helix-receptor interactions: the Bcl-2 family inhibitor BH3I-1 potently inhibits p53/hDM2Chem. Comm46, 8020-8022 (2010).

Epigenetics & DNA Damage Detection

8)  Badran, AH; Furman, JL; Ma, AS; Comi, TJ; Porter, JR; Ghosh, I.  Evaluating the Global CpG Methylation Status of Native DNA Utilizing a Bipartite Split-Luciferase Sensor.  Anal. Chem. 83, 7151-7157 (2011).

9)  Porter, JR; Lockwood, SH; Segal, DJ; Ghosh, I.  Seeing genetic and epigenetic information without DNA denaturation using sequence-enabled reassembly (SEER).  Methods Mol. Biol649, 365-82 (2010).

10) Yu, YN; Blair, S; Gillespie, D; Jensen, R; Myszka, D; Badran, AH; Ghosh, I; Chagovetz, A.   Direct DNA Methylation Profiling Using Methyl Binding Domain Proteins.  Anal. Chem. 82, 5012-5019 (2010).

11) Furman, JL; Badran, AH; Shen, SY; Stains, CI; Hannallah, J; Segal, DJ; Ghosh, I. Systematic evaluation of split-fluorescent proteins for the direct detection of native and methylated DNA.  Bioorg. Med. Chem. Lett. 19, 3748-3751 (2009).

12) Porter, JR; Stains, CI; Segal, DJ; Ghosh, I. Split beta-lactamase sensor for the sequence-specific detection of DNA methylationAnal. Chem79, 6702-6708 (2007).

13) Stains, CI; Furman, JL; Segal, DJ; Ghosh, I.  Site-specific detection of DNA methylation utilizing mCpG-SEER.  J. Am. Chem. Soc. 128, 9761-9765 (2006).

14) Furman, JL; Mok, PW; Badran, AH; Ghosh, I.  Turn-On DNA Damage Sensors for the Direct Detection of 8-Oxoguanine and Photoproducts in Native DNA.  J. Am. Chem. Soc133, 12518-12527 (2011).

15) Furman, JL; Mok, PW; Shen, SY; Stains, CI; Ghosh, I.  A turn-on split-luciferase sensor for the direct detection of poly(ADP-ribose) as a marker for DNA repair and cell death.  Chem. Comm47, 397-399 (2011).

16) Ghosh, I; Stains, CI; Ooi, AT; Segal, DJ. Direct detection of double-stranded DNA: molecular methods and applications for DNA diagnosticsMol. Biosys. 2,  551-560 (2006).

17) Ooi, AT; Stains, CI; Ghosh, I; Segal, DJ.  Sequence-enabled reassembly of beta-lactamase (SEER-LAC): A sensitive method for the detection of double stranded DNA.  Biochemistry45, 3620-3625 (2006).

18) Stains, CI; Porter, JR; Ooi, AT; Segal, DJ; Ghosh, I.  DNA sequence-enabled reassembly of the green fluorescent proteinJ. Am. Chem. Soc.  127, 10782-10783 (2005).

Proteases & Other Targets

19) Shekhawat, SS; Campbell, ST; Ghosh, I.  A Comprehensive Panel of Turn-On Caspase Biosensors for Investigating Caspase Specificity and Caspase Activation Pathways.  ChemBiochem 12,2353-2364 (2011).

20) Shekhawat, SS; Porter, JR; Sriprasad, A; Ghosh, I.  An Autoinhibited Coiled-Coil Design Strategy for Split-Protein Protease Sensors.  J. Am. Chem. Soc. 131, 15284-15290 (2009).

21) Furman, JL; Badran, AH; Ajulo, O; Porter, JR; Stains, CI; Segal, DJ; Ghosh, I.  Toward a General Approach for RNA-Templated Hierarchical Assembly of Split-Proteins.  J. Am. Chem. Soc. 132, 11692-11701 (2010).