|
|
|
Kinases play a fundamental role in human biology, controlling many cellular processes through protein phosphorylation. Disregulation of kinase function leads to diseases like cancer, inflammation and diabetes. Kinases are very similar in their active site architecture, which leads to cross-reactivity amongst kinase targeted small-molecule inhibitors. It is important to determine the affinity and selectivity of such inhibitors to minimize off-target effects while optimizing desired selectivity profiles. Luceome's KinaseSeeker™ is a high throughput screening and profiling service for evaluation of compounds against a panel of kinases. |
|
Luceome's KinaseSeeker™ assay technology utilizes the luciferase fragment complementation, in which re-assembly of a split-protein leads to a functional luciferase enzyme and bioluminescence. In Luceome's proprietary assay, the reassembly of the luciferase fragments is dependent upon the interaction of a kinase with a small molecule. This assay thus directly measures the ability of a specific kinase to bind to a customer-provided small molecule inhibitor. Since the assay is luminescence based, background interference and hence false positives from test compounds is minimized during kinase-profiling. |
|
Luceome's KinaseSeeker™ service utilizes a novel method for low-cost screening and profiling of potential drug candidates against a panel of kinases, in a near cellular environment.
Features and Benefits
- Luminescence detection: Low false positives from fluorescent compounds
- Sensitive: Low Background; Broad dynamic range
- Binding Assay: Identify active site directed and allosteric inhibitors
- Homogeneous: Portable; Adaptable to high-throughput
- Economical: 50 kinase panel profiling - $300 (Volume Discounts Available)
- Choose the kinases you want
- Rapid turnaround of data: < 1 week
- Data kept confidential
|
|
|
Click here to request a quote
- High-throughput Screening
- Customized Kinase Profiling
- IC50 determination
|
|
|
Luceome provides a custom assay development service to clients who wish to use the luciferase fragment complementation assay for screening inhibitors in-house. We will tailor the assay to fit your profiling needs. For an initial Feasibility Assessment or to Request a Quote, please contact us at Email:
This e-mail address is being protected from spambots. You need JavaScript enabled to view it
Phone: (520) 495-0161 KinaseSeeker™ and KinaseLite™ are trademarks of Luceome Biotechnologies. Luceome's assay technology is covered by pending patents. |
|
|
|
|
|
